![]() Researchers in immunology, pharmacology, medicine. The machine uses Sony software to setup and analyze sorting and flow cytometry experiments.įlowJo software is also available on the FACSCanto II Flow Cytometer for advanced data analysis. FlowJo was conceived in the laboratory to address this need and easily analyze flow cytometry data. This machine can sort into a variety of formats including tubes, 96 well plates and 384 well plates, offering flexibility for downstream analysis. In reality, flow data is rarely normal and. In a perfect world, our data would be normally distributed and in that case means, median and mode are all equal. MFI is often used without explanation, to abbreviate either arithmetic mean, geometric mean, or median fluorescence intensity. Every user will still need an account on the FlowJo Portal using your email address. The first point of confusion is born from the name itself. If provides increased flexibility to users by allowing them to manage their own seat on the license through the website. The machine uses disposable microfluidic sorting chips that are available in three sizes (70 µm, 100 µm and 130 µm) meaning a diverse range of cell sizes can be sorted (bacteria, archaea and mammalian cells). Portal is FlowJo’s web-based site license management tool. This course will address the use of plug-ins to extend the functionality of FlowJo, including Sunburst. FlowJo can accommodate high dimension, high throughput flow, or mass cytometry data. The machine is equipped with four excitation lasers (488nm, 405nm, 638nm and 561nm) and six emission filters that allow detection of a diverse range of fluorescent proteins and labels. Description: In this advanced FlowJo cytometry webinar participants will learn how to move beyond the basics of the application to conduct in-depth analyses. If the Serial Number dialog box does not come up automatically when FlowJo is launched, open it from under the FlowJo menu (in OSX) or the Apple menu (in OS9). An individual copy of FlowJo is normally 2695.00 with the academic discount the site license brings that cost down to 285/year for each license that can. The Sony SH800S Cell sorter is for performing flow cytometry and fluorescence-activated cell sorting (FACs) experiments. The hardware address of the computer will show up at the bottom of the Serial Number dialog box. FlowJo has launched their new username and password based licensing system called FlowJo Portal the University of Washington has access to site licensing through the Cell Analysis Facility.
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